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fix perm diluent 1  (Cytek Biosciences)


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    Structured Review

    Cytek Biosciences fix perm diluent 1
    Fix Perm Diluent 1, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fix+perm+diluent+1/Foxp3+%2F+Transcription+Factor+Fix%2FPerm+Diluent/us11591297-279-41-34
    Average 93 stars, based on 28 article reviews
    fix perm diluent 1 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Residue:

    Article Title: Indole alkaloid compound having immune checkpoint inhibitory action
    Article Snippet: .. The residue was suspended in phosphate buffered saline (supplemented with 1% bovine serum albumin (BSA), without calcium and magnesium) (1% BSA/PBS) to adjust the concentration of cells to 5-10×106 cells/mL. (3) To 5 ml tube, each 50 μL of the cell suspension was added. (4) Foxp3/Transcription Factor Fix/Perm Concentrate (4×) (Cat. No. TNB-1020-L050) (Solution A) in FOXP3/Transcription Factor Staining kit (TONBO biosciences) was diluted with Foxp3/Transcription Factor Fix/Perm Diluent (1×) (Cat. No. TNB-1022-L160) (for dilution of Solution A) to prepare 1× Solution A. (5) 1 mL of 1× Solution A was added to each tube and mixed by gentle pipetting. ..

    Saline:

    Article Title: Indole alkaloid compound having immune checkpoint inhibitory action
    Article Snippet: .. The residue was suspended in phosphate buffered saline (supplemented with 1% bovine serum albumin (BSA), without calcium and magnesium) (1% BSA/PBS) to adjust the concentration of cells to 5-10×106 cells/mL. (3) To 5 ml tube, each 50 μL of the cell suspension was added. (4) Foxp3/Transcription Factor Fix/Perm Concentrate (4×) (Cat. No. TNB-1020-L050) (Solution A) in FOXP3/Transcription Factor Staining kit (TONBO biosciences) was diluted with Foxp3/Transcription Factor Fix/Perm Diluent (1×) (Cat. No. TNB-1022-L160) (for dilution of Solution A) to prepare 1× Solution A. (5) 1 mL of 1× Solution A was added to each tube and mixed by gentle pipetting. ..

    Concentration Assay:

    Article Title: Indole alkaloid compound having immune checkpoint inhibitory action
    Article Snippet: .. The residue was suspended in phosphate buffered saline (supplemented with 1% bovine serum albumin (BSA), without calcium and magnesium) (1% BSA/PBS) to adjust the concentration of cells to 5-10×106 cells/mL. (3) To 5 ml tube, each 50 μL of the cell suspension was added. (4) Foxp3/Transcription Factor Fix/Perm Concentrate (4×) (Cat. No. TNB-1020-L050) (Solution A) in FOXP3/Transcription Factor Staining kit (TONBO biosciences) was diluted with Foxp3/Transcription Factor Fix/Perm Diluent (1×) (Cat. No. TNB-1022-L160) (for dilution of Solution A) to prepare 1× Solution A. (5) 1 mL of 1× Solution A was added to each tube and mixed by gentle pipetting. ..

    Suspension:

    Article Title: Indole alkaloid compound having immune checkpoint inhibitory action
    Article Snippet: .. The residue was suspended in phosphate buffered saline (supplemented with 1% bovine serum albumin (BSA), without calcium and magnesium) (1% BSA/PBS) to adjust the concentration of cells to 5-10×106 cells/mL. (3) To 5 ml tube, each 50 μL of the cell suspension was added. (4) Foxp3/Transcription Factor Fix/Perm Concentrate (4×) (Cat. No. TNB-1020-L050) (Solution A) in FOXP3/Transcription Factor Staining kit (TONBO biosciences) was diluted with Foxp3/Transcription Factor Fix/Perm Diluent (1×) (Cat. No. TNB-1022-L160) (for dilution of Solution A) to prepare 1× Solution A. (5) 1 mL of 1× Solution A was added to each tube and mixed by gentle pipetting. ..

    Staining:

    Article Title: Indole alkaloid compound having immune checkpoint inhibitory action
    Article Snippet: .. The residue was suspended in phosphate buffered saline (supplemented with 1% bovine serum albumin (BSA), without calcium and magnesium) (1% BSA/PBS) to adjust the concentration of cells to 5-10×106 cells/mL. (3) To 5 ml tube, each 50 μL of the cell suspension was added. (4) Foxp3/Transcription Factor Fix/Perm Concentrate (4×) (Cat. No. TNB-1020-L050) (Solution A) in FOXP3/Transcription Factor Staining kit (TONBO biosciences) was diluted with Foxp3/Transcription Factor Fix/Perm Diluent (1×) (Cat. No. TNB-1022-L160) (for dilution of Solution A) to prepare 1× Solution A. (5) 1 mL of 1× Solution A was added to each tube and mixed by gentle pipetting. ..

    Article Title: Compound having PD-L1 expression-suppressing action
    Article Snippet: The tubes were then allowed to stand at room temperature for 15 minutes. (4) Anti-CD274-PC7 antibody (BECKMAN COULTER, Cat. No. A78884) or mouse IgG1-PC7 antibody (BECKMAN COULTER, 737662) as control was added in each tube in an amount of 10 μL, and the tubes were gently stirred. .. The tubes were then allowed to stand in the dark on an ice bath for 30 minutes. (5) Foxp3/Transcription Factor Fix/Perm Concentrate (4×) (Cat. No. TNB-1020-L050) (A solution) supplied in FOXP3/Transcription Factor Staining kit (TONBO biosciences) was diluted in Foxp3/Transcription Factor Fix/Perm Diluent (1×) (Cat. No. TNB-1022-L160) (A dilution solution) to prepare a 1×A solution. (6) The 1×A solution was added into each tube in an amount of 1 mL, and the tubes were gently pipetted and stirred. .. The tubes were then allowed to stand in the dark at room temperature for 30 minutes and centrifuged at 300×g for 5 minutes, and then the supernatants therein were removed. (7) 2 mL of 1% BSA/PBS− was added into each tube and the tubes were gently stirred and centrifuged at 300×g for 5 minutes, and then the supernatants therein were removed. (8) The cells therein were suspended in 0.5 mL of 1% BSA/PBS- and measured with a flow cytometer (Sony: Cell Sorter SH800) within 2 hours.

    Gentle:

    Article Title: Indole alkaloid compound having immune checkpoint inhibitory action
    Article Snippet: .. The residue was suspended in phosphate buffered saline (supplemented with 1% bovine serum albumin (BSA), without calcium and magnesium) (1% BSA/PBS) to adjust the concentration of cells to 5-10×106 cells/mL. (3) To 5 ml tube, each 50 μL of the cell suspension was added. (4) Foxp3/Transcription Factor Fix/Perm Concentrate (4×) (Cat. No. TNB-1020-L050) (Solution A) in FOXP3/Transcription Factor Staining kit (TONBO biosciences) was diluted with Foxp3/Transcription Factor Fix/Perm Diluent (1×) (Cat. No. TNB-1022-L160) (for dilution of Solution A) to prepare 1× Solution A. (5) 1 mL of 1× Solution A was added to each tube and mixed by gentle pipetting. ..



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